In Vitro Cytotoxic Analysis of Ethanolic Extract from Leaves of Kalanchoe blossfeldiana Poelln (Crassulaceae)
Amanda Alany F. L. Cruz1, José Eduardo dos S. Gomes1, Vinícius Xavier F. Andrade1, Vicente Gabriel G. de Macedo1, Ana Paula C. da Silva1, Anderson P. de Lima1, José W. Almeida-Bezerra2*, Raquel Furtado dos S. Moura2, Francildo dos S. Silva2, Paula Patrícia M. Cordeiro2, Ademar M. Filho2, Maria I. Rocha2, Anita Oliveira Brito P. B. Martins2, Cícera Natalia F. L. Gondim2, Rizelle de O. Barros3, Joycy Francely S. dos Santos2, Anna Lídia N. Varela2,3, Lucas dos S. Sa2, José Walber G. Castro1, Raíra Justino O. Costa1
1Dr. Lea o Sampaio University Center, Juazeiro do Norte – CE, Brazil.
2Regional University of Cariri, Crato – CE, Brazil
3Federal University of Cariri – Crato – CE, Brazil.
*Corresponding Author: Prof. Dr. Jose Weverton Almeida-Bezerra, Department of Biological Chemistry, Regional University of Cariri, 63105-000, Crato, CE, Brazil.
https://doi.org/10.58624/SVOAMB.2025.06.023
Received: November 15, 2025
Published: December 12, 2025
Citation: Cruz AAFL, Gomes JEDS, Andrade VXF, Macedo VGG, Silva APC, Lima AP, Almeida-Bezerra JW, Moura RFS, Silva FS, Cordeiro PPM, Filho AM, Rocha MI, Martins AOBPB, Gondim CNFL, Barros RO, Santos JFS, Varela ALN, Sa LS, Castro JWG, Costa RJO. In Vitro Cytotoxic Analysis of Ethanolic Extract from Leaves of Kalanchoe blossfeld iana Poelln (Crassulaceae). SVOA Microbiology 2025, 6:6, 204-209. doi:10.58624/SVOAMB.2025.06.023
Abstract
The idea of harmlessness “Natura doesn’t hurt” is quite common, due to the lack of knowledge of the floral biology of some species, especially ornamental ones, as these studies are recent in Brazil. The present study aimed to perform an in vitro cytotoxicity analysis of the leaves of the ornamental plant Kalanchoe blossfeldiana Poelln on human red blood cells. After approval of the research project by the Brazilian Ethics Committee, identification of the plant by an Herbarium and selection of volunteers for the research by signing the TCLE and TCPE, the plant was purchased from a local florist and taken to the laboratory, since it is a plant grown in florists. The leaves were washed and 200 g were standardized, followed by grinding, maceration of the leaves in 95% ethanol and evaporation of the solvent. The cytotoxicity test was performed on red blood cells, in which the extract was diluted in six different concentrations and incubated with red blood cell washes for 1 hour. Immediately after, the samples were centrifuged and the absorbance of the supernatant was measured at 540 nm by spectrophotometry. From the results, the hemolysis percentages and statistical analysis were calculated using GraphPad Prism 7.0® software, with results at p<0.01 considered statistically significant. Hemolysis was observed in all concentrations compared to the control and that this hemolysis percentage increased proportionally to the extract concentration. It is concluded that the leaf extract shows signs of toxicity, revealing that care must be taken when cultivating the species, so that its leaves are not ingested.
Keywords: Plant Poisoning; Statistical Analysis; Kalanchoe.
